WBIR-TV reported that the Tennessee Wildlife Resources Agency, which had ownership of the snakes, gave the order Monday to euthanize them.
TWRA confiscated more than 50 poisonous snakes from pastor Andrew Hamblin's Tabernacle Church of God in Campbell County in November. A grand jury exonerated Hamblin earlier this month. Hamblin appeared last fall on the National Geographic Channel reality show "Snake Salvation."
The snakes were taken to the Knoxville Zoo, where officials said 39 died of multiple parasites and pathogens and the rest were believed to be infected also.